Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable Vaccine
Identifieur interne : 001303 ( Main/Exploration ); précédent : 001302; suivant : 001304Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable Vaccine
Auteurs : Jose M. Jimenez-Guarde O [Espagne] ; Jose A. Regla-Nava [Espagne] ; Jose L. Nieto-Torres [Espagne] ; Marta L. Dediego [Espagne] ; Carlos Casta O-Rodriguez [Espagne] ; Raul Fernandez-Delgado [Espagne] ; Stanley Perlman [États-Unis] ; Luis Enjuanes [Espagne]Source :
- PLoS Pathogens [ 1553-7366 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Virus du SRAS.
- immunologie : Vaccins antiviraux, Vaccins atténués, Vaccins synthétiques, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- Animaux, Cellules cultivées, Femelle, Souris, Souris de lignée BALB C, Virulence.
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Vaccines, Attenuated, Vaccines, Synthetic, Viral Vaccines.
- growth & development : SARS Virus.
- immunology : SARS Virus.
- pathogenicity : SARS Virus.
- Animals, Cells, Cultured, Female, Mice, Mice, Inbred BALB C, Virulence.
Abstract
A SARS-CoV lacking the full-length E gene (SARS-CoV-∆E) was attenuated and an effective vaccine. Here, we show that this mutant virus regained fitness after serial passages in cell culture or
Url:
DOI: 10.1371/journal.ppat.1005215
PubMed: 26513244
PubMed Central: 4626112
Affiliations:
- Espagne, États-Unis
- Communauté de Madrid, Iowa
- Iowa City, Madrid
- Université autonome de Madrid, Université de l'Iowa
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Le document en format XML
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Regla-Nava</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName><orgName type="university">Université autonome de Madrid</orgName></affiliation></author><author><name sortKey="Nieto Torres, Jose L" sort="Nieto Torres, Jose L" uniqKey="Nieto Torres J" first="Jose L." last="Nieto-Torres">Jose L. Nieto-Torres</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName><orgName type="university">Université autonome de Madrid</orgName></affiliation></author><author><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L." last="Dediego">Marta L. Dediego</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName><orgName type="university">Université autonome de Madrid</orgName></affiliation></author><author><name sortKey="Casta O Rodriguez, Carlos" sort="Casta O Rodriguez, Carlos" uniqKey="Casta O Rodriguez C" first="Carlos" last="Casta O-Rodriguez">Carlos Casta O-Rodriguez</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName><orgName type="university">Université autonome de Madrid</orgName></affiliation></author><author><name sortKey="Fernandez Delgado, Raul" sort="Fernandez Delgado, Raul" uniqKey="Fernandez Delgado R" first="Raul" last="Fernandez-Delgado">Raul Fernandez-Delgado</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName><orgName type="university">Université autonome de Madrid</orgName></affiliation></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><affiliation wicri:level="4"><nlm:aff id="aff002"><addr-line>Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Iowa, Iowa City, Iowa</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName><orgName type="university">Université autonome de Madrid</orgName></affiliation></author></analytic><series><title level="j">PLoS Pathogens</title><idno type="ISSN">1553-7366</idno><idno type="eISSN">1553-7374</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>SARS Virus (growth & development)</term><term>SARS Virus (immunology)</term><term>SARS Virus (pathogenicity)</term><term>Vaccines, Attenuated (immunology)</term><term>Vaccines, Synthetic (immunology)</term><term>Viral Vaccines (immunology)</term><term>Virulence</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Femelle</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins atténués (immunologie)</term><term>Vaccins synthétiques (immunologie)</term><term>Virulence</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Vaccines, Attenuated</term><term>Vaccines, Synthetic</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Vaccins antiviraux</term><term>Vaccins atténués</term><term>Vaccins synthétiques</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Femelle</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A SARS-CoV lacking the full-length E gene (SARS-CoV-∆E) was attenuated and an effective vaccine. Here, we show that this mutant virus regained fitness after serial passages in cell culture or <italic>in vivo</italic>, resulting in the partial duplication of the membrane gene or in the insertion of a new sequence in gene 8a, respectively. The chimeric proteins generated in cell culture increased virus fitness <italic>in vitro</italic> but remained attenuated in mice. In contrast, during SARS-CoV-∆E passage in mice, the virus incorporated a mutated variant of 8a protein, resulting in reversion to a virulent phenotype. When the full-length E protein was deleted or its PDZ-binding motif (PBM) was mutated, the revertant viruses either incorporated a novel chimeric protein with a PBM or restored the sequence of the PBM on the E protein, respectively. Similarly, after passage in mice, SARS-CoV-∆E protein 8a mutated, to now encode a PBM, and also regained virulence. These data indicated that the virus requires a PBM on a transmembrane protein to compensate for removal of this motif from the E protein. To increase the genetic stability of the vaccine candidate, we introduced small attenuating deletions in E gene that did not affect the endogenous PBM, preventing the incorporation of novel chimeric proteins in the virus genome. In addition, to increase vaccine biosafety, we introduced additional attenuating mutations into the nsp1 protein. Deletions in the carboxy-terminal region of nsp1 protein led to higher host interferon responses and virus attenuation. Recombinant viruses including attenuating mutations in E and nsp1 genes maintained their attenuation after passage <italic>in vitro</italic> and <italic>in vivo</italic>. Further, these viruses fully protected mice against challenge with the lethal parental virus, and are therefore safe and stable vaccine candidates for protection against SARS-CoV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name></author><author><name sortKey="Netland, J" uniqKey="Netland J">J Netland</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name></author><author><name sortKey="Oberste, Ms" uniqKey="Oberste M">MS Oberste</name></author><author><name sortKey="Monroe, Ss" uniqKey="Monroe S">SS Monroe</name></author><author><name sortKey="Nix, Wa" uniqKey="Nix W">WA Nix</name></author><author><name sortKey="Campganoli, R" uniqKey="Campganoli R">R Campganoli</name></author><author><name sortKey="Icenogle, Jp" uniqKey="Icenogle J">JP Icenogle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S van der Werf</name></author><author><name sortKey="Brodt, Hr" uniqKey="Brodt H">HR Brodt</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S Becker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Tsoi, Hw" uniqKey="Tsoi H">HW Tsoi</name></author><author><name sortKey="Wong, Bh" uniqKey="Wong B">BH Wong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name></author><author><name sortKey="Yu, M" uniqKey="Yu M">M Yu</name></author><author><name sortKey="Ren, W" uniqKey="Ren W">W Ren</name></author><author><name sortKey="Smith, C" uniqKey="Smith C">C Smith</name></author><author><name sortKey="Epstein, Jh" uniqKey="Epstein J">JH Epstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name></author><author><name sortKey="Poon, Rw" uniqKey="Poon R">RW Poon</name></author><author><name sortKey="Wong, Bh" uniqKey="Wong B">BH Wong</name></author><author><name sortKey="Tsoi, Hw" uniqKey="Tsoi H">HW Tsoi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dominguez, Sr" uniqKey="Dominguez S">SR Dominguez</name></author><author><name sortKey="O Shea, Tj" uniqKey="O Shea T">TJ O'Shea</name></author><author><name sortKey="Oko, Lm" uniqKey="Oko L">LM Oko</name></author><author><name sortKey="Holmes, Kv" uniqKey="Holmes K">KV Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A Bermingham</name></author><author><name sortKey="Chand, M" uniqKey="Chand M">M Chand</name></author><author><name sortKey="Brown, C" uniqKey="Brown C">C Brown</name></author><author><name sortKey="Aarons, E" uniqKey="Aarons E">E Aarons</name></author><author><name sortKey="Tong, C" uniqKey="Tong C">C Tong</name></author><author><name sortKey="Langrish, C" uniqKey="Langrish C">C Langrish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Ito, N" uniqKey="Ito N">N Ito</name></author><author><name sortKey="Tseng, Ct" uniqKey="Tseng C">CT Tseng</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shen, S" uniqKey="Shen S">S Shen</name></author><author><name sortKey="Lin, Ps" uniqKey="Lin P">PS Lin</name></author><author><name sortKey="Chao, Yc" uniqKey="Chao Y">YC Chao</name></author><author><name sortKey="Zhang, A" uniqKey="Zhang A">A Zhang</name></author><author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name></author><author><name sortKey="Lim, Sg" uniqKey="Lim S">SG Lim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, M" uniqKey="Qiu M">M Qiu</name></author><author><name sortKey="Shi, Y" uniqKey="Shi Y">Y Shi</name></author><author><name sortKey="Guo, Z" uniqKey="Guo Z">Z Guo</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name></author><author><name sortKey="He, R" uniqKey="He R">R He</name></author><author><name sortKey="Chen, R" uniqKey="Chen R">R Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saif, Lj" uniqKey="Saif L">LJ Saif</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buchholz, Uj" uniqKey="Buchholz U">UJ Buchholz</name></author><author><name sortKey="Bukreyev, A" uniqKey="Bukreyev A">A Bukreyev</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L Yang</name></author><author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name></author><author><name sortKey="Murphy, Br" uniqKey="Murphy B">BR Murphy</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name></author><author><name sortKey="Deming, D" uniqKey="Deming D">D Deming</name></author><author><name sortKey="Sheahan, T" uniqKey="Sheahan T">T Sheahan</name></author><author><name sortKey="Baric, R" uniqKey="Baric R">R Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, J" uniqKey="Huang J">J Huang</name></author><author><name sortKey="Cao, Y" uniqKey="Cao Y">Y Cao</name></author><author><name sortKey="Du, J" uniqKey="Du J">J Du</name></author><author><name sortKey="Bu, X" uniqKey="Bu X">X Bu</name></author><author><name sortKey="Ma, R" uniqKey="Ma R">R Ma</name></author><author><name sortKey="Wu, C" uniqKey="Wu C">C Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Channappanavar, R" uniqKey="Channappanavar R">R Channappanavar</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Torres, J" uniqKey="Torres J">J Torres</name></author><author><name sortKey="Parthasarathy, K" uniqKey="Parthasarathy K">K Parthasarathy</name></author><author><name sortKey="Lin, X" uniqKey="Lin X">X Lin</name></author><author><name sortKey="Saravanan, R" uniqKey="Saravanan R">R Saravanan</name></author><author><name sortKey="Liu, Dx" uniqKey="Liu D">DX Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Verdia Baguena, C" uniqKey="Verdia Baguena C">C Verdia-Baguena</name></author><author><name sortKey="Nieto Torres, Jl" uniqKey="Nieto Torres J">JL Nieto-Torres</name></author><author><name sortKey="Alcaraz, A" uniqKey="Alcaraz A">A Alcaraz</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML Dediego</name></author><author><name sortKey="Torres, J" uniqKey="Torres J">J Torres</name></author><author><name sortKey="Aguilella, Vm" uniqKey="Aguilella V">VM Aguilella</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Verdia Baguena, C" uniqKey="Verdia Baguena C">C Verdia-Baguena</name></author><author><name sortKey="Nieto Torres, Jl" uniqKey="Nieto Torres J">JL Nieto-Torres</name></author><author><name sortKey="Alcaraz, A" uniqKey="Alcaraz A">A Alcaraz</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML Dediego</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Aguilella, Vm" uniqKey="Aguilella V">VM Aguilella</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nieto Torres, Jl" uniqKey="Nieto Torres J">JL Nieto-Torres</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML Dediego</name></author><author><name sortKey="Verdia Baguena, C" uniqKey="Verdia Baguena C">C Verdia-Baguena</name></author><author><name sortKey="Jimenez Guarde O, Jm" uniqKey="Jimenez Guarde O J">JM Jimenez-Guardeño</name></author><author><name sortKey="Regla Nava, Ja" uniqKey="Regla Nava J">JA Regla-Nava</name></author><author><name sortKey="Fernandez Delgado, R" uniqKey="Fernandez Delgado R">R Fernandez-Delgado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jimenez Guarde O, Jm" uniqKey="Jimenez Guarde O J">JM Jimenez-Guardeño</name></author><author><name sortKey="Nieto Torres, Jl" uniqKey="Nieto Torres J">JL Nieto-Torres</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Regla Nava, Ja" uniqKey="Regla Nava J">JA Regla-Nava</name></author><author><name sortKey="Fernandez Delgado, R" uniqKey="Fernandez Delgado R">R Fernandez-Delgado</name></author><author><name sortKey="Casta O Rodriguez, C" uniqKey="Casta O Rodriguez C">C Castaño-Rodriguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Teoh, Kt" uniqKey="Teoh K">KT Teoh</name></author><author><name sortKey="Siu, Yl" uniqKey="Siu Y">YL Siu</name></author><author><name sortKey="Chan, Wl" uniqKey="Chan W">WL Chan</name></author><author><name sortKey="Schluter, Ma" uniqKey="Schluter M">MA Schluter</name></author><author><name sortKey="Liu, Cj" uniqKey="Liu C">CJ Liu</name></author><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hung, Ay" uniqKey="Hung A">AY Hung</name></author><author><name sortKey="Sheng, M" uniqKey="Sheng M">M Sheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munz, M" uniqKey="Munz M">M Munz</name></author><author><name sortKey="Hein, J" uniqKey="Hein J">J Hein</name></author><author><name sortKey="Biggin, Pc" uniqKey="Biggin P">PC Biggin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gerek, Zn" uniqKey="Gerek Z">ZN Gerek</name></author><author><name sortKey="Keskin, O" uniqKey="Keskin O">O Keskin</name></author><author><name sortKey="Ozkan, Sb" uniqKey="Ozkan S">SB Ozkan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Javier, Rt" uniqKey="Javier R">RT Javier</name></author><author><name sortKey="Rice, Ap" uniqKey="Rice A">AP Rice</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name></author><author><name sortKey="Jackson, Jp" uniqKey="Jackson J">JP Jackson</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name></author><author><name sortKey="Sheahan, T" uniqKey="Sheahan T">T Sheahan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Netland, J" uniqKey="Netland J">J Netland</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author><author><name sortKey="Fett, C" uniqKey="Fett C">C Fett</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name></author><author><name sortKey="Nieto Torres, Jl" uniqKey="Nieto Torres J">JL Nieto-Torres</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fett, C" uniqKey="Fett C">C Fett</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Regla Nava, Ja" uniqKey="Regla Nava J">JA Regla-Nava</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name></author><author><name sortKey="Almazan, F" uniqKey="Almazan F">F Almazan</name></author><author><name sortKey="Rejas, Mt" uniqKey="Rejas M">MT Rejas</name></author><author><name sortKey="Lamirande, E" uniqKey="Lamirande E">E Lamirande</name></author><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Pewe, L" uniqKey="Pewe L">L Pewe</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name></author><author><name sortKey="Rejas, Mt" uniqKey="Rejas M">MT Rejas</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Graham, Rl" uniqKey="Graham R">RL Graham</name></author><author><name sortKey="Donaldson, Ef" uniqKey="Donaldson E">EF Donaldson</name></author><author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lambert, Ph" uniqKey="Lambert P">PH Lambert</name></author><author><name sortKey="Liu, M" uniqKey="Liu M">M Liu</name></author><author><name sortKey="Siegrist, Ca" uniqKey="Siegrist C">CA Siegrist</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liao, Cl" uniqKey="Liao C">CL Liao</name></author><author><name sortKey="Lai, Mmc" uniqKey="Lai M">MMC Lai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Regla Nava, Ja" uniqKey="Regla Nava J">JA Regla-Nava</name></author><author><name sortKey="Jimenez Guarde O, Jm" uniqKey="Jimenez Guarde O J">JM Jimenez-Guardeño</name></author><author><name sortKey="Nieto Torres, Jl" uniqKey="Nieto Torres J">JL Nieto-Torres</name></author><author><name sortKey="Gallagher, Tm" uniqKey="Gallagher T">TM Gallagher</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kamitani, W" uniqKey="Kamitani W">W Kamitani</name></author><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name></author><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Lokugamage, K" uniqKey="Lokugamage K">K Lokugamage</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author><author><name sortKey="Ito, N" uniqKey="Ito N">N Ito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kamitani, W" uniqKey="Kamitani W">W Kamitani</name></author><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name></author><author><name sortKey="Lokugamage, Kg" uniqKey="Lokugamage K">KG Lokugamage</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Lokugamage, Kg" uniqKey="Lokugamage K">KG Lokugamage</name></author><author><name sortKey="Rozovics, Jm" uniqKey="Rozovics J">JM Rozovics</name></author><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name></author><author><name sortKey="Semler, Bl" uniqKey="Semler B">BL Semler</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tanaka, T" uniqKey="Tanaka T">T Tanaka</name></author><author><name sortKey="Kamitani, W" uniqKey="Kamitani W">W Kamitani</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Matsuura, Y" uniqKey="Matsuura Y">Y Matsuura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tohya, Y" uniqKey="Tohya Y">Y Tohya</name></author><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name></author><author><name sortKey="Kamitani, W" uniqKey="Kamitani W">W Kamitani</name></author><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Lokugamage, K" uniqKey="Lokugamage K">K Lokugamage</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name></author><author><name sortKey="Ramirez, Si" uniqKey="Ramirez S">SI Ramirez</name></author><author><name sortKey="Lokugamage, Kg" uniqKey="Lokugamage K">KG Lokugamage</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name></author><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Lokugamage, K" uniqKey="Lokugamage K">K Lokugamage</name></author><author><name sortKey="Kamitani, W" uniqKey="Kamitani W">W Kamitani</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author><author><name sortKey="Tseng, Ct" uniqKey="Tseng C">CT Tseng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wathelet, Mg" uniqKey="Wathelet M">MG Wathelet</name></author><author><name sortKey="Orr, M" uniqKey="Orr M">M Orr</name></author><author><name sortKey="Frieman, Mb" uniqKey="Frieman M">MB Frieman</name></author><author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author><author><name sortKey="Bian, G" uniqKey="Bian G">G Bian</name></author><author><name sortKey="Tu, J" uniqKey="Tu J">J Tu</name></author><author><name sortKey="Xing, Y" uniqKey="Xing Y">Y Xing</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zust, R" uniqKey="Zust R">R Zust</name></author><author><name sortKey="Cervantes Barragan, L" uniqKey="Cervantes Barragan L">L Cervantes-Barragan</name></author><author><name sortKey="Kuri, T" uniqKey="Kuri T">T Kuri</name></author><author><name sortKey="Blakqori, G" uniqKey="Blakqori G">G Blakqori</name></author><author><name sortKey="Weber, F" uniqKey="Weber F">F Weber</name></author><author><name sortKey="Ludewig, B" uniqKey="Ludewig B">B Ludewig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lei, L" uniqKey="Lei L">L Lei</name></author><author><name sortKey="Ying, S" uniqKey="Ying S">S Ying</name></author><author><name sortKey="Baojun, L" uniqKey="Baojun L">L Baojun</name></author><author><name sortKey="Yi, Y" uniqKey="Yi Y">Y Yi</name></author><author><name sortKey="Xiang, H" uniqKey="Xiang H">H Xiang</name></author><author><name sortKey="Wenli, S" uniqKey="Wenli S">S Wenli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name></author><author><name sortKey="Bredenbeek, Pj" uniqKey="Bredenbeek P">PJ Bredenbeek</name></author><author><name sortKey="Dobbe, Jc" uniqKey="Dobbe J">JC Dobbe</name></author><author><name sortKey="Thiel, V" uniqKey="Thiel V">V Thiel</name></author><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J Ziebuhr</name></author><author><name sortKey="Poon, Llm" uniqKey="Poon L">LLM Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Den Born, E" uniqKey="Van Den Born E">E van den Born</name></author><author><name sortKey="Gultyaev, Ap" uniqKey="Gultyaev A">AP Gultyaev</name></author><author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zu Iga, S" uniqKey="Zu Iga S">S Zuñiga</name></author><author><name sortKey="Sola, I" uniqKey="Sola I">I Sola</name></author><author><name sortKey="Alonso, S" uniqKey="Alonso S">S Alonso</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Regla Nava, Ja" uniqKey="Regla Nava J">JA Regla-Nava</name></author><author><name sortKey="Nieto Torres, Jl" uniqKey="Nieto Torres J">JL Nieto-Torres</name></author><author><name sortKey="Jimenez Guarde O, Jm" uniqKey="Jimenez Guarde O J">JM Jimenez-Guardeño</name></author><author><name sortKey="Fernandez Delgado, R" uniqKey="Fernandez Delgado R">R Fernandez-Delgado</name></author><author><name sortKey="Fett, C" uniqKey="Fett C">C Fett</name></author><author><name sortKey="Casta O Rodriguez, C" uniqKey="Casta O Rodriguez C">C Castaño-Rodriguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, Nl" uniqKey="Tang N">NL Tang</name></author><author><name sortKey="Chan, Pk" uniqKey="Chan P">PK Chan</name></author><author><name sortKey="Wong, Ck" uniqKey="Wong C">CK Wong</name></author><author><name sortKey="To, Kf" uniqKey="To K">KF To</name></author><author><name sortKey="Wu, Ak" uniqKey="Wu A">AK Wu</name></author><author><name sortKey="Sung, Ym" uniqKey="Sung Y">YM Sung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smits, Sl" uniqKey="Smits S">SL Smits</name></author><author><name sortKey="De Lang, A" uniqKey="De Lang A">A de Lang</name></author><author><name sortKey="Van Den Brand, Jm" uniqKey="Van Den Brand J">JM van den Brand</name></author><author><name sortKey="Leijten, Lm" uniqKey="Leijten L">LM Leijten</name></author><author><name sortKey="Van, Iwf" uniqKey="Van I">IWF van</name></author><author><name sortKey="Eijkemans, Mj" uniqKey="Eijkemans M">MJ Eijkemans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Jsm" uniqKey="Peiris J">JSM Peiris</name></author><author><name sortKey="Lai, St" uniqKey="Lai S">ST Lai</name></author><author><name sortKey="Poon, Llm" uniqKey="Poon L">LLM Poon</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Yam, Lyc" uniqKey="Yam L">LYC Yam</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Nieto Torres, Jl" uniqKey="Nieto Torres J">JL Nieto-Torres</name></author><author><name sortKey="Regla Nava, Ja" uniqKey="Regla Nava J">JA Regla-Nava</name></author><author><name sortKey="Jimenez Guarde O, Jm" uniqKey="Jimenez Guarde O J">JM Jimenez-Guardeño</name></author><author><name sortKey="Fernandez Delgado, R" uniqKey="Fernandez Delgado R">R Fernandez-Delgado</name></author><author><name sortKey="Fett, C" uniqKey="Fett C">C Fett</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frieman, Mb" uniqKey="Frieman M">MB Frieman</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Morrison, Te" uniqKey="Morrison T">TE Morrison</name></author><author><name sortKey="Whitmore, A" uniqKey="Whitmore A">A Whitmore</name></author><author><name sortKey="Funkhouser, W" uniqKey="Funkhouser W">W Funkhouser</name></author><author><name sortKey="Ward, Jm" uniqKey="Ward J">JM Ward</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sheahan, T" uniqKey="Sheahan T">T Sheahan</name></author><author><name sortKey="Morrison, Te" uniqKey="Morrison T">TE Morrison</name></author><author><name sortKey="Funkhouser, W" uniqKey="Funkhouser W">W Funkhouser</name></author><author><name sortKey="Uematsu, S" uniqKey="Uematsu S">S Uematsu</name></author><author><name sortKey="Akira, S" uniqKey="Akira S">S Akira</name></author><author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuo, L" uniqKey="Kuo L">L Kuo</name></author><author><name sortKey="Masters, Ps" uniqKey="Masters P">PS Masters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuo, L" uniqKey="Kuo L">L Kuo</name></author><author><name sortKey="Masters, Ps" uniqKey="Masters P">PS Masters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fu, Ks" uniqKey="Fu K">KS Fu</name></author><author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ballesteros, Ml" uniqKey="Ballesteros M">ML Ballesteros</name></author><author><name sortKey="Sanchez, Cm" uniqKey="Sanchez C">CM Sanchez</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eckerle, Ld" uniqKey="Eckerle L">LD Eckerle</name></author><author><name sortKey="Becker, Mm" uniqKey="Becker M">MM Becker</name></author><author><name sortKey="Halpin, Ra" uniqKey="Halpin R">RA Halpin</name></author><author><name sortKey="Li, K" uniqKey="Li K">K Li</name></author><author><name sortKey="Venter, E" uniqKey="Venter E">E Venter</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuo, L" uniqKey="Kuo L">L Kuo</name></author><author><name sortKey="Masters, Ps" uniqKey="Masters P">PS Masters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, Ds" uniqKey="Song D">DS Song</name></author><author><name sortKey="Yang, Js" uniqKey="Yang J">JS Yang</name></author><author><name sortKey="Oh, Js" uniqKey="Oh J">JS Oh</name></author><author><name sortKey="Han, Jh" uniqKey="Han J">JH Han</name></author><author><name sortKey="Park, Bk" uniqKey="Park B">BK Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woods, Rd" uniqKey="Woods R">RD Woods</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sanchez, Cm" uniqKey="Sanchez C">CM Sanchez</name></author><author><name sortKey="Izeta, A" uniqKey="Izeta A">A Izeta</name></author><author><name sortKey="Sanchez Morgado, Jm" uniqKey="Sanchez Morgado J">JM Sanchez-Morgado</name></author><author><name sortKey="Alonso, S" uniqKey="Alonso S">S Alonso</name></author><author><name sortKey="Sola, I" uniqKey="Sola I">I Sola</name></author><author><name sortKey="Balasch, M" uniqKey="Balasch M">M Balasch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ye, F" uniqKey="Ye F">F Ye</name></author><author><name sortKey="Zhang, M" uniqKey="Zhang M">M Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tonikian, R" uniqKey="Tonikian R">R Tonikian</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Sazinsky, Sl" uniqKey="Sazinsky S">SL Sazinsky</name></author><author><name sortKey="Currell, B" uniqKey="Currell B">B Currell</name></author><author><name sortKey="Yeh, Jh" uniqKey="Yeh J">JH Yeh</name></author><author><name sortKey="Reva, B" uniqKey="Reva B">B Reva</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name></author><author><name sortKey="Deming, D" uniqKey="Deming D">D Deming</name></author><author><name sortKey="Paddock, Cd" uniqKey="Paddock C">CD Paddock</name></author><author><name sortKey="Cheng, A" uniqKey="Cheng A">A Cheng</name></author><author><name sortKey="Yount, B" uniqKey="Yount B">B Yount</name></author><author><name sortKey="Vogel, L" uniqKey="Vogel L">L Vogel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Cc" uniqKey="Chen C">CC Chen</name></author><author><name sortKey="Kruger, J" uniqKey="Kruger J">J Kruger</name></author><author><name sortKey="Sramala, I" uniqKey="Sramala I">I Sramala</name></author><author><name sortKey="Hsu, Hj" uniqKey="Hsu H">HJ Hsu</name></author><author><name sortKey="Henklein, P" uniqKey="Henklein P">P Henklein</name></author><author><name sortKey="Chen, Ym" uniqKey="Chen Y">YM Chen</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Chiu, Rw" uniqKey="Chiu R">RW Chiu</name></author><author><name sortKey="Chim, Ss" uniqKey="Chim S">SS Chim</name></author><author><name sortKey="Tong, Yk" uniqKey="Tong Y">YK Tong</name></author><author><name sortKey="Fung, Ks" uniqKey="Fung K">KS Fung</name></author><author><name sortKey="Chan, Pk" uniqKey="Chan P">PK Chan</name></author><author><name sortKey="Zhao, Gp" uniqKey="Zhao G">GP Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melik, W" uniqKey="Melik W">W Melik</name></author><author><name sortKey="Ellencrona, K" uniqKey="Ellencrona K">K Ellencrona</name></author><author><name sortKey="Wigerius, M" uniqKey="Wigerius M">M Wigerius</name></author><author><name sortKey="Hedstrom, C" uniqKey="Hedstrom C">C Hedstrom</name></author><author><name sortKey="Elvang, A" uniqKey="Elvang A">A Elvang</name></author><author><name sortKey="Johansson, M" uniqKey="Johansson M">M Johansson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ellencrona, K" uniqKey="Ellencrona K">K Ellencrona</name></author><author><name sortKey="Syed, A" uniqKey="Syed A">A Syed</name></author><author><name sortKey="Johansson, M" uniqKey="Johansson M">M Johansson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Spaller, Mr" uniqKey="Spaller M">MR Spaller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bisht, H" uniqKey="Bisht H">H Bisht</name></author><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name></author><author><name sortKey="Vogel, L" uniqKey="Vogel L">L Vogel</name></author><author><name sortKey="Bukreyev, A" uniqKey="Bukreyev A">A Bukreyev</name></author><author><name sortKey="Collins, Pl" uniqKey="Collins P">PL Collins</name></author><author><name sortKey="Murphy, Br" uniqKey="Murphy B">BR Murphy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name></author><author><name sortKey="Mcauliffe, J" uniqKey="Mcauliffe J">J McAuliffe</name></author><author><name sortKey="Vogel, L" uniqKey="Vogel L">L Vogel</name></author><author><name sortKey="Fahle, G" uniqKey="Fahle G">G Fahle</name></author><author><name sortKey="Fischer, S" uniqKey="Fischer S">S Fischer</name></author><author><name sortKey="Tatti, K" uniqKey="Tatti K">K Tatti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Almazan, F" uniqKey="Almazan F">F Almazan</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Galan, C" uniqKey="Galan C">C Galan</name></author><author><name sortKey="Escors, D" uniqKey="Escors D">D Escors</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name></author><author><name sortKey="Ortego, J" uniqKey="Ortego J">J Ortego</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nieto Torres, Jl" uniqKey="Nieto Torres J">JL Nieto-Torres</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name></author><author><name sortKey="Jimenez Guarde O, Jm" uniqKey="Jimenez Guarde O J">JM Jimenez-Guardeño</name></author><author><name sortKey="Regla Nava, Ja" uniqKey="Regla Nava J">JA Regla-Nava</name></author><author><name sortKey="Llorente, M" uniqKey="Llorente M">M Llorente</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bustin, Sa" uniqKey="Bustin S">SA Bustin</name></author><author><name sortKey="Benes, V" uniqKey="Benes V">V Benes</name></author><author><name sortKey="Garson, Ja" uniqKey="Garson J">JA Garson</name></author><author><name sortKey="Hellemans, J" uniqKey="Hellemans J">J Hellemans</name></author><author><name sortKey="Huggett, J" uniqKey="Huggett J">J Huggett</name></author><author><name sortKey="Kubista, M" uniqKey="Kubista M">M Kubista</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kallberg, M" uniqKey="Kallberg M">M Kallberg</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author><author><name sortKey="Peng, J" uniqKey="Peng J">J Peng</name></author><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z Wang</name></author><author><name sortKey="Lu, H" uniqKey="Lu H">H Lu</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Espagne</li><li>États-Unis</li></country><region><li>Communauté de Madrid</li><li>Iowa</li></region><settlement><li>Iowa City</li><li>Madrid</li></settlement><orgName><li>Université autonome de Madrid</li><li>Université de l'Iowa</li></orgName></list><tree><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Jimenez Guarde O, Jose M" sort="Jimenez Guarde O, Jose M" uniqKey="Jimenez Guarde O J" first="Jose M." last="Jimenez-Guarde O">Jose M. Jimenez-Guarde O</name></region><name sortKey="Casta O Rodriguez, Carlos" sort="Casta O Rodriguez, Carlos" uniqKey="Casta O Rodriguez C" first="Carlos" last="Casta O-Rodriguez">Carlos Casta O-Rodriguez</name><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L." last="Dediego">Marta L. Dediego</name><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name><name sortKey="Fernandez Delgado, Raul" sort="Fernandez Delgado, Raul" uniqKey="Fernandez Delgado R" first="Raul" last="Fernandez-Delgado">Raul Fernandez-Delgado</name><name sortKey="Nieto Torres, Jose L" sort="Nieto Torres, Jose L" uniqKey="Nieto Torres J" first="Jose L." last="Nieto-Torres">Jose L. Nieto-Torres</name><name sortKey="Regla Nava, Jose A" sort="Regla Nava, Jose A" uniqKey="Regla Nava J" first="Jose A." last="Regla-Nava">Jose A. Regla-Nava</name></country><country name="États-Unis"><region name="Iowa"><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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